17 - 21 Jun 2019

ESDR/EADV Summer School in Molecular Tools in Dermatological and Pigment Cell Research

This summer school, held in English, is specifically aimed at dermatology residents/trainees and at PhD students and postdocs affiliated to dermatological research focusing on the melanocyte lineage including hypo/hyper-pigmentation of the skin and hair and on melanoma with limited experience in experimental research and in molecular biology.

 

The workshop will comprise a mixture of research presentations, and interactive and practical sessions. An electronic and printed booklet of methods and protocols presented during the course will be given to participants at the beginning of the course.

 

Main Topics will include:

* Establishment of the melanocyte lineage

* Homeostasis of the melanocyte lineage

* Congenital and acquired hypo/hyper-pigmentation

* Melanoma

 

Specific Topics will cover:

* Establishment of the melanocyte lineage, in vivo and in vitro imaging - Melanosome

* Congenital Giant nevus, Vitiligo - Preclinical development in vitiligo

* Autophagy and pigmentation, Single cells analysis, Genetic of pigmentation and melanoma

* Mechanisms of melanomagenesis, Microbiota and melanomagenesis

* Interaction Melanocyte – Microenvironment, Clinical update on melanoma

 

Practical courses will include:

* Establishment of melanocytes and melanoma cells in culture from mouse models

* Overexpression and KD (transfection and infection) of genes in cell lines

* Read out: auto-fluorescence, immunofluorescence, WB, RT-PCR, and RNAscope

  • Localization of melanoblasts in the embryo – labeling (Dct::LacZ, ZEG)

 

Prior to the course the participants will prepare a presentation and a poster (size A0 portrait) to be presented and discussed during the course (7' presentation + 2' Q&A). This presentation will be either based on their own research project or from a relevant scientific article from the field that they will critically apprise in case they have not yet performed any research.

The EADV would like to thank the Curie Institute, the University of Paris Sud and the University of Paris Saclay for gracisouly providing us with the auditorium and the laboratory facilities for this course.

Where

Orsay, France

Terms

EADV Resident courses are for dermatology and venereology residents (both EADV members and non EADV members).

Selected candidates will receive free registration and the educational material which was presented during the course. EADV also offers accommodation, catering and a social event during the course.

EADV members will benefit from an educational grant, to be eligible for the grant annual membership dues must be settled one month prior to the course.

The selection of candidates is made based on CVs, year of residency and the amount of courses previously attended.

The registration deadline will close at 23h59 (GMT+1) the day before the registration deadline.

Programme

Monday 17 June 2019

  • 08:30 / 09:00 Welcome and overview of the course in dermatological and pigment cell research   -   Lionel LARUE
  • 09:00 / 09:40 Establishment of the melanocyte lineage   -   Véronique DELMAS
  • 09:40 / 10:40 Participant Presentation (1-5)   -   Chairperson Franck Gesbert
  • 10:45 / 11:15 Coffee Break and Posters
  • 11:15 / 11:55 Migration of melanoblasts - in vivo imaging   -   Laura MACHESKY
  • 11:55 / 12:35 Establishment of melanocytes in culture   -   Lionel LARUE
  • 12:35 / 13:50 Lunch and Posters
  • 14:00 / 14:30 Organization of the practical session and laboratory safety
  • 14:30 / 18:30 Practical session (#1): Infection/transfection of mouse/human melanocyte/melanoma cell lines. Preparation of protein/RNA
  • 18:30 / 20:00 Welcome Drink @ Institut Curie

Tuesday 18 June 2019

  • 09:00 / 09:40 Pigmentation and melanosomes   -   Cedric DELEVOYE
  • 09:40 / 10:40 Participant Presentation (6-10)   -   Chairperson Zackie Aktary
  • 10:45 / 11:15 Coffee Break and Posters
  • 11:15 / 11:55 Hypopigmentation   -   Julien SENESCHAL
  • 11:55 / 12:35 Genetic manipulation & mouse pigment models   -   Lionel LARUE
  • 12:35 / 13:50 Lunch and Posters
  • 14:00 / 18:30 Practical session (#2): Follow up of transfected and infected cells. Western blot and RT-PCR. In vitro imaging.

Wednesday 19 June 2019

  • 09:00 / 09:40 Hyperpigmentation   -   Julien SENESCHAL
  • 09:40 / 10:40 Participant Presentation (11-15)   -   Chairperson Franck Gesbert
  • 10:45 / 11:15 Coffee Break and Posters
  • 11:15 / 11:55 Congenital melanocytic naevi   -   Veronica KINSLER
  • 11:55 / 12:35 Crispr Cas9, melanocytes and melanoma   -   Eirikur STEINGRIMSSON
  • 12:35 / 13:50 Lunch and Posters
  • 14:00 / 18:30 Practical session (#3): Follow up of transfected and infected cells. Western blot and RT-qPCR. In vitro imaging. Isolation and staining of mouse embryos

Thursday 20 June 2019

  • 09:00 / 09:40 Autophagy in melanocytes and keratinocytes   -   Erwin TSCHACHLER
  • 09:40 / 10:40 Participant Presentation (16-20)   -   Chairperson Zackie AKTARY
  • 10:45 / 11:15 Coffee Break and Posters
  • 11:15 / 11:55 Genetics of melanoma   -   Eirikur STEINGRIMSSON
  • 11:55 / 12:35 Mechanisms of melanomagenesis   -   Colin GODING
  • 12:35 / 13:50 Lunch and Posters
  • 14:00 / 18:00 Practical session #4 (last session): Follow up of transfected and infected cells. Western blot analysis. Follow up of stained embryos (whole mount and histological analysis)
  • 19:30 / 23:00 Social Event

Friday 21 June 2019

  • 09:00 / 09:40 Melanoma, metabolism and microbiota   -   Colin GODING
  • 09:40 / 10:20 Intratumoral heterogeneity and single cells analysis   -   Irwin DAVIDSON
  • 10:20 / 11:00 Coffee Break
  • 11:00 / 11:40 Searching efficiently in PubMed   -   Veronique DELMAS
  • 11:40 / 12:00 Closing discussion
Download agenda